AN ENZYME IN MAST CELLS WITH PROPERTIES LIKE CHYMOTRYPSIN
نویسندگان
چکیده
منابع مشابه
An Enzyme in Mast Cells with Properties like Chymotrypsin
Mast cells contain an enzyme which hydrolyzes 3-chloroacetoxy-2-naphthoic acid anilide. By using highly purified mast cells isolated by differential centrifugation in high density sucrose solutions we have been able to study this enzymatic activity in more detail. The enzyme has properties similar to those of chymotrypsin: Chymotrypsin will hydrolyze the histochemical substrate, and the chymotr...
متن کاملA human lung mast cell chymotrypsin-like enzyme. Identification and partial characterization.
We have used a high performance liquid chromatography assay, which detects chymotryptic cleavage of the phe8-his9 bond of angiotensin I to yield angiotensin II, in order to examine human lung mast cells for the presence of chymotryptic activity. Mast cells, purified from human lung by enzymatic dispersion, countercurrent elutriation, and Percoll gradient centrifugation, were lysed or challenged...
متن کاملHistochemical demonstration of chymotrypsin like serine esterases in mucosal mast cells in four species including man.
Serine esterases were detected in the granules of mucosal mast cells from rat, mouse, sheep, and man. Successful demonstration of enzyme activity required brief fixation (6 h) of tissues in 4% paraformaldehyde. Staining with naphthol AS-D chloroacetate produced an intense red reaction product in intraepithelial mucosal mast cells (globule leucocytes) and mucosal mast cells within the lamina pro...
متن کاملMAST CELLS IN INFLAMMATORY AURAL POLYPS
This study investigated the presence of mast cells in inflammatory aural polyps using light microscopy. The number of mast cells was increased in all examined cases and varied from 2.33 to 41.54 per mm2• The cells were located most commonly around small blood vessels. Occurrence of mast cells was not different in various types of polyps, but their density was severely increased in active in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1959
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.110.3.451